IMMUNOFLUORESCENT LOCALIZATION OF VITAMIN D-DEPENDENT CALCIUM-BINDING PROTEIN
نویسندگان
چکیده
منابع مشابه
Vitamin D-dependent Calcium-binding Protein
A method of purification of a vitamin D-dependent calciumbinding protein from chick intestinal mucosa is described. The purification procedure involved the use of ammonium sulfate precipitation, gel filtration on Sephadex G-100, and preparative disc electrophoresis. The assay system for calcium-binding protein is based upon the competition between calcium-binding protein and an ion exchange res...
متن کاملImmunocytochemical localization of the vitamin D-dependent calcium binding protein in chick duodenum
The vitamin D-dependent calcium binding protein (CaBP) of chick duodenum has been localized by immunocytochemistry and by radioimmunoassay. Light microscopically, CaBP was seen to be present in the absorptive cells of the villi while in other cell types of the villi and the crypts, including goblet cells and endocrine cells, no CaBP was seen. At the electron microscopic level, CaBP was shown to...
متن کاملIntestinal membrane calcium-binding protein. Vitamin D-dependent membrane component of the intestinal calcium transport mechanism.
A particulate fraction of rat intestinal mucosal homogenates, termed the "calcium-binding complex," contains three vitamin D-dependent activities: calcium binding of high affinity, calcium-dependent adenosine triphosphatase, and p-nitrophenylphosphatase. These particulate activities vary concordantly with intestinal calcium transport, suggesting that they represent membrane components of the tr...
متن کاملPlacental calcium binding protein in rats. Apparent identity with vitamin D-dependent calcium binding protein from rat intestine.
Rat placenta contains a calcium binding protein (CaBP) which is closely related or identical with the vitamin Ddependent CaBP in rat intestine. Placental CaBP was detected using antiserum prepared in rabbits against the intestinal form of the protein purified as previously described (Bruns, M. E. H., Fliesher, E. B., and Avioli, L. V. (1977) J. Biol. Chem. 252, 4145-4150). The antiserum was mon...
متن کاملAnalysis of vitamin D-dependent calcium-binding protein messenger ribonucleic acid expression in mice lacking the vitamin D receptor.
To investigate the roles of the receptor-dependent actions of 1,25-dihydroxyvitamin D3 [1,25-(OH)2D3] in the regulation of vitamin D-dependent calcium-binding proteins (calbindin-D), the messenger RNA (mRNA) levels of calbindin-D9k and -28k were examined in vitamin D receptor (VDR)-ablated mice and control littermates. In VDR-ablated mice, calbindin-D9k mRNA was dramatically reduced in the inte...
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ژورنال
عنوان ژورنال: Journal of Histochemistry & Cytochemistry
سال: 1970
ISSN: 0022-1554,1551-5044
DOI: 10.1177/18.2.107